A simple, sensitive, specific, spectrophotometric method was developed for the detection of Phenytoin Sodium (PS). The optimum condition for the analysis of the drug was studied. PS was subjected to stress degradation under different conditions recommended by the International Conference on Harmonization (ICH). The samples so prepared were used for degradation studies by using the developed method. The absorbance maxima (λmax) of the PS was found to be 202.40 nm. The method reveals high sensitivity, with linearity in the 2 to 10 µg/ml range. The lower limit of detection was found to be 0.05 µg/ml and the limit of quantification was found to be 0.13 µg/ml. All the calibration curves demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient higher than 0.99. The regression equation of the curve was Y=0.0963x + 0.2137. The precision of the method was found to be 0.739 ± 0.002733. The %recovery was found to be 100.234. The sample solution was stable for 7 days in refrigerator.
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